A Dual Color ELISPOT Assay
نویسنده
چکیده
The ELISPOT assay is considered by many to be a gold standard for monitoring specific cellular immune responses. It can detect single cells secreting molecules of interest (1-3). This assay is highly sensitive, quantitative, easy to use and amenable to high throughput (4). Until recently assays using chromophores to detect the secretion of cytokines from single cells was limited by the difficulty of detecting mixed color spots (5;6).
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